iTRAQ protein profile analysis of Citrus sinensis roots in response to long-term boron-deficiency.
Identifieur interne : 000C65 ( Main/Exploration ); précédent : 000C64; suivant : 000C66iTRAQ protein profile analysis of Citrus sinensis roots in response to long-term boron-deficiency.
Auteurs : Lin-Tong Yang [République populaire de Chine] ; Yi-Ping Qi ; Yi-Bin Lu ; Peng Guo ; Wen Sang ; Hui Feng ; Hong-Xing Zhang ; Li-Song ChenSource :
- Journal of proteomics [ 1876-7737 ] ; 2013.
English descriptors
- KwdEn :
- MESH :
- chemical , deficiency : Boron.
- chemical , metabolism : Boron, Plant Proteins.
- genetics : Citrus sinensis.
- metabolism : Citrus sinensis, Plant Roots, Seedlings.
- methods : Proteomics.
- Down-Regulation, Transcriptome, Up-Regulation.
Abstract
Seedlings of Citrus sinensis were fertilized with boron (B)-deficient (0μM H3BO3) or -sufficient (10μM H3BO3) nutrient solution for 15weeks. Thereafter, iTRAQ analysis was employed to compare the abundances of proteins from B-deficient and -sufficient roots. In B-deficient roots, 164 up-regulated and 225 down-regulated proteins were identified. These proteins were grouped into the following functional categories: protein metabolism, nucleic acid metabolism, stress responses, carbohydrate and energy metabolism, cell transport, cell wall and cytoskeleton metabolism, biological regulation and signal transduction, and lipid metabolism. The adaptive responses of roots to B-deficiency might include following several aspects: (a) decreasing root respiration; (b) improving the total ability to scavenge reactive oxygen species (ROS); and (c) enhancing cell transport. The differentially expressed proteins identified by iTRAQ are much larger than those detected using 2D gel electrophoresis, and many novel B-deficiency-responsive proteins involved in cell transport, biological regulation and signal transduction, stress responses and other metabolic processes were identified in this work. Our results indicate remarkable metabolic flexibility of citrus roots, which may contribute to the survival of B-deficient plants. This represents the most comprehensive analysis of protein profiles in response to B-deficiency.
DOI: 10.1016/j.jprot.2013.04.025
PubMed: 23628855
Affiliations:
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Institute of Horticultural Plant Physiology, Biochemistry and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Resources and Environmental Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Horticultural Plant Physiology, Biochemistry and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou 350002</wicri:regionArea><placeName><settlement type="city">Fuzhou</settlement><region type="province">Fujian</region></placeName></affiliation></author><author><name sortKey="Qi, Yi Ping" sort="Qi, Yi Ping" uniqKey="Qi Y" first="Yi-Ping" last="Qi">Yi-Ping Qi</name></author><author><name sortKey="Lu, Yi Bin" sort="Lu, Yi Bin" uniqKey="Lu Y" first="Yi-Bin" last="Lu">Yi-Bin Lu</name></author><author><name sortKey="Guo, Peng" sort="Guo, Peng" uniqKey="Guo P" first="Peng" last="Guo">Peng Guo</name></author><author><name sortKey="Sang, Wen" sort="Sang, Wen" uniqKey="Sang W" first="Wen" last="Sang">Wen Sang</name></author><author><name sortKey="Feng, Hui" sort="Feng, Hui" uniqKey="Feng H" first="Hui" last="Feng">Hui Feng</name></author><author><name sortKey="Zhang, Hong Xing" sort="Zhang, Hong Xing" uniqKey="Zhang H" first="Hong-Xing" last="Zhang">Hong-Xing Zhang</name></author><author><name sortKey="Chen, Li Song" sort="Chen, Li Song" uniqKey="Chen L" first="Li-Song" last="Chen">Li-Song Chen</name></author></analytic><series><title level="j">Journal of proteomics</title><idno type="eISSN">1876-7737</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Boron (deficiency)</term><term>Boron (metabolism)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (metabolism)</term><term>Down-Regulation</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (metabolism)</term><term>Proteomics (methods)</term><term>Seedlings (metabolism)</term><term>Transcriptome</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Boron</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Boron</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term><term>Plant Roots</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Down-Regulation</term><term>Transcriptome</term><term>Up-Regulation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Seedlings of Citrus sinensis were fertilized with boron (B)-deficient (0μM H3BO3) or -sufficient (10μM H3BO3) nutrient solution for 15weeks. Thereafter, iTRAQ analysis was employed to compare the abundances of proteins from B-deficient and -sufficient roots. In B-deficient roots, 164 up-regulated and 225 down-regulated proteins were identified. These proteins were grouped into the following functional categories: protein metabolism, nucleic acid metabolism, stress responses, carbohydrate and energy metabolism, cell transport, cell wall and cytoskeleton metabolism, biological regulation and signal transduction, and lipid metabolism. The adaptive responses of roots to B-deficiency might include following several aspects: (a) decreasing root respiration; (b) improving the total ability to scavenge reactive oxygen species (ROS); and (c) enhancing cell transport. The differentially expressed proteins identified by iTRAQ are much larger than those detected using 2D gel electrophoresis, and many novel B-deficiency-responsive proteins involved in cell transport, biological regulation and signal transduction, stress responses and other metabolic processes were identified in this work. Our results indicate remarkable metabolic flexibility of citrus roots, which may contribute to the survival of B-deficient plants. This represents the most comprehensive analysis of protein profiles in response to B-deficiency.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Fujian</li></region><settlement><li>Fuzhou</li></settlement></list><tree><noCountry><name sortKey="Chen, Li Song" sort="Chen, Li Song" uniqKey="Chen L" first="Li-Song" last="Chen">Li-Song Chen</name><name sortKey="Feng, Hui" sort="Feng, Hui" uniqKey="Feng H" first="Hui" last="Feng">Hui Feng</name><name sortKey="Guo, Peng" sort="Guo, Peng" uniqKey="Guo P" first="Peng" last="Guo">Peng Guo</name><name sortKey="Lu, Yi Bin" sort="Lu, Yi Bin" uniqKey="Lu Y" first="Yi-Bin" last="Lu">Yi-Bin Lu</name><name sortKey="Qi, Yi Ping" sort="Qi, Yi Ping" uniqKey="Qi Y" first="Yi-Ping" last="Qi">Yi-Ping Qi</name><name sortKey="Sang, Wen" sort="Sang, Wen" uniqKey="Sang W" first="Wen" last="Sang">Wen Sang</name><name sortKey="Zhang, Hong Xing" sort="Zhang, Hong Xing" uniqKey="Zhang H" first="Hong-Xing" last="Zhang">Hong-Xing Zhang</name></noCountry><country name="République populaire de Chine"><region name="Fujian"><name sortKey="Yang, Lin Tong" sort="Yang, Lin Tong" uniqKey="Yang L" first="Lin-Tong" last="Yang">Lin-Tong Yang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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